Q: Is BAM®S different from fluorescent antibodies and other related technologies?

It is different. BAM ® S relies on the inherent fluorescence (also known as "autofluorescence") emitted by the fluorophores in the cells of microorganisms, so there is no need to prepare samples to detect microorganisms. This is in contrast to the fluorescent dye-based technology, in which in order to detect microorganisms, the sample needs to be treated with reagents to emit a specific fluorescent signal. This process is embodied in common techniques such as ATP biofluorescence detection and flow cytometry detection.

Q: Can BAM ® S identify microorganisms?

BAM ® S cannot identify microorganisms, because the inherent fluorescent groups used for detection are widely present in microorganisms, so the specificity to specific genus and/or species is excluded. Some customers use microbial identification methods (such as RT-PCR, gas chromatography, etc.) to respond to excessive microbial standards. This method can still be used with BAM ® S; at the same time, after BAM ® S detects an alarm, it can use the MV interconnection function to trigger the planktonic bacteria sampler to immediately collect contaminated air samples for subsequent bacterial identification and analysis. In this feedback feedback should be used, timely detection and response are the most important factors (for example, you cannot identify microorganisms and perform root cause analysis of undetected microbial deviations).

Question 1

What is the ideal use of BAM ® S?

Accepted Answer

When timely information on critical environments is very important, BAM ® S is your ideal choice. BAM ® S sets the speed standard for rapid microbial detection, provides continuous real-time data about the environment, and enables users to detect, react and prevent pollution with a sensitivity that cannot be achieved by traditional methods. The most common BAM ® S applications involve critical environments in the pharmaceutical and other life science industries, such as clean room monitoring, including monitoring of key locations such as aseptic filling lines, sterility test kits/isolators, and RABS areas; other specialized applications Including personnel training, compressed gas testing, process simulation (such as medium filling), root cause investigation (such as the source of contamination of building molds), engineering verification (such as determining the best HVAC settings), and risk assessment/process improvement. Many of these scenarios provide value by providing a continuous risk reduction mechanism, but other benefits include reduced cleaning cycles to save energy, improved resource allocation by reducing sampling frequency, and improved process understanding

Question 2

Compared with traditional plate culture methods and other "rapid microbiology methods" (RMMs), in which aspects are BAM ® S more popular?

Accepted Answer

BAM ® S is superior to traditional plate culture methods and other rapid methods in the following aspects:

1) About the detection frequency: continuous sampling, trend analysis can be performed;

2) Time to obtain results: Real-time results facilitate the identification of pollution sources;

3) When a positive result or exceeding the standard occurs: sampling of microbial planktonic bacteria can be carried out in time, and central control samples can be carried out in time

Sampling and sampling of relevant personnel in order to assess the impact on the product in time, which is conducive to the investigation of the source of pollution;

4) In terms of cost: no reagents are required, and labor costs are minimal.

Question 3

Many instruments can detect particle size, some instruments can detect fluorescence, and some instruments can even detect particle size and fluorescence at the same time. Why must the size and fluorescence of each particle be measured at the same time?

Accepted Answer

If the measurement of particle characteristics comes from different instruments, or even at different times from the same instrument, it is difficult to use advanced deterministic processing algorithms to link the measurement results. Such algorithms and related measures need to achieve the highest sensitivity while minimizing the possible impact of electronic "noise", which is crucial, for example, in determining whether the detected particles have inert or microbial properties. BAM &reg; S uses a dedicated algorithm to analyze the size and fluorescence information of each particle in real time to provide deterministic results.

Question 4

Is BAM®S different from fluorescent antibodies and other related technologies?

Accepted Answer

It is different. BAM &reg; S relies on the inherent fluorescence (also known as "autofluorescence") emitted by the fluorophores in the cells of microorganisms, so there is no need to prepare samples to detect microorganisms. This is in contrast to the fluorescent dye-based technology, in which in order to detect microorganisms, the sample needs to be treated with reagents to emit a specific fluorescent signal. This process is embodied in common techniques such as ATP biofluorescence detection and flow cytometry detection.

Question 5

Can BAM ® S identify microorganisms?

Accepted Answer

BAM &reg; S cannot identify microorganisms, because the inherent fluorescent groups used for detection are widely present in microorganisms, so the specificity to specific genus and/or species is excluded. Some customers use microbial identification methods (such as RT-PCR, gas chromatography, etc.) to respond to excessive microbial standards. This method can still be used with BAM &reg; S; at the same time, after BAM &reg; S detects an alarm, it can use the MV interconnection function to trigger the planktonic bacteria sampler to immediately collect contaminated air samples for subsequent bacterial identification and analysis. In this feedback feedback should be used, timely detection and response are the most important factors (for example, you cannot identify microorganisms and perform root cause analysis of undetected microbial deviations).

Question 6

Does BAM ® S distinguish between dead and live microbial particles?

Accepted Answer

The answer to this question depends on how much inherent fluorescence capacity is left in the killed microorganisms. If a large number of fluorophores are intact, dead cells may continue to emit enough fluorescence to be classified as microorganisms. However, if the microorganism has been dead for a period of time, or is killed by a chemical technique that destroys the fluorophore in the cell, the remaining fluorescence will be below the threshold required by BAM &reg; S to count the particles as microorganisms.

BAMS FAQ

What is the ideal use of BAM ® S?

Compared with traditional plate culture methods and other "rapid microbiology methods" (RMMs), in which aspects are BAM ® S more popular?

Many instruments can detect particle size, some instruments can detect fluorescence, and some instruments can even detect particle size and fluorescence at the same time. Why must the size and fluorescence of each particle be measured at the same time?

Is BAM®S different from fluorescent antibodies and other related technologies?

Can BAM ® S identify microorganisms?

Does BAM ® S distinguish between dead and live microbial particles?

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